CRISPR-Cas9

英文解释

CRISPR-Cas9 is an efficient, fast, easy technique for achieving knock-out gene in the cell. The mechanism has three steps: recognition, cleavage, and repair. The designed sgRNA directs Cas-9 and recognizes the target sequence. The Cas-9 nuclease is activated and makes double-stranded breaks (DSBs) when sgRNA recognizes the target sequence.

中文解释

CRISPR-Cas9 是一种高效、快速、简便的细胞基因敲除技术。其机制分为三个步骤：识别、切割和修复。所设计的向导 RNA (sgRNA) 可引导 Cas-9 并识别目标序列。当 sgRNA 识别目标序列后，Cas-9 核酸酶会被激活并产生双链断裂 (DSB)。

参考文献

[1]. Asmamaw M, et al. Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing. Biologics. 2021 Aug 21;15:353-361.