Fluorescence in situ hybridization (FISH)
发布时间:2023-03-22 22:08:39
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Fluorescence in situ hybridization (FISH)
荧光原位杂交
英文解释
Fluorescence in situ hybridization (FISH) is a macromolecule recognition technology. FISH uses DNA fragments incorporated with fluorophore-coupled nucleotides as probes, and examine the presence or absence of complementary sequences in fixed cells or tissues under a fluorescent microscope.
中文解释
荧光原位杂交 (FISH) 是一种大分子识别技术。FISH 使用掺入荧光团-核苷酸偶联物的 DNA 片段作为探针,可在荧光显微镜下检查固定细胞或组织中是否存在互补序列。
参考文献
[1]. Cui C, et al. Fluorescence In situ Hybridization: Cell-Based Genetic Diagnostic and Research Applications. Front Cell Dev Biol. 2016 Sep 5;4:89.