T4 DNA Ligase

T4 DNA 连接酶

英文解释

T4 DNA ligase can repair single-strand nicks of single-stranded or double-stranded nucleic acid, and catalyze phosphodiester bond between the 5'-P and 3'-OH ends of the sticky-ended or flat-ended double-stranded DNA or RNA with the help of cofactor ATP.

中文解释

T4 DNA 连接酶可以修补核酸单链或双链的单链缺口(single-strand nicks)，可以借助辅助因子ATP，催化粘端或平端双链 DNA 或 RNA 的 5’-末端的磷酸和 3’-末端的羟基以磷酸二酯键结合。

参考文献

[1]. Gansauge MT, et al. Single-stranded DNA library preparation from highly degraded DNA using T4 DNA ligase. Nucleic Acids Res. 2017 Jun 2;45(10):e79.