Background:
(±)9(10)-EpOME is the 9,10-cis epoxide of linoleic acid, generated by neutrophils during the oxidative burst.1 It has been recovered from the lungs of hyperoxic rats and from humans with acute respiratory distress syndrome.2 Mitochondrial dysfunction is the main feature of (±)9(10)-EpOME cytotoxicity, which may be due to the diol metabolites as well as the parent epoxide.3,4
(±)9(10)-EpOME MaxSpec®sup>? standard is a quantitative grade standard of (±)9(10)-EpOME that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This (±)9(10)-EpOME MaxSpec®sup>? standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.
1.Hayakawa, M., Sugiyama, S., Takamura, T., et al.Neutrophils biosynthesize leukotoxin, 9,10-epoxy-12-octadecenoateBiochem. Biophys. Res. Commun.137(1)424-430(1986) 2.Ozawa, T., Hayakawa, M., Takamura, T., et al.Biosynthesis of leukotoxin, 9,10-epoxy-12 octadecenoate, by leukocytes in lung lavages of rat after exposure to hyperoxiaBiochem. Biophys. Res. Commun.134(3)1071-1078(1986) 3.Kosaka, K., Suzuki, K., Hayakawa, M., et al.Leukotoxin, a linoleate epoxide: Its implication in the late death of patients with extensive burnsMol. Cell. Biochem.139(2)141-148(1994) 4.Moran, J.H., Weise, R., Schnellmann, R.G., et al.Cytotoxicity of linoleic acid diols to renal proximal tubular cellsToxicol. Appl. Pharmacol.146(1)53-59(1997)
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