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FM-red(PSH-red)是一种发红光且对环境敏感的探针,用于选择性检测和标记蛋白硫醇。FM-red可用于在活细胞和体内蛋白质巯基的成像。FM-red也可用于测量硫氧还蛋白(Trx)的氧化还原状态。
FM-red (PSH-red) is a red-emitting and environment-sensitive probe for selectively detecting and labeling protein thiols. FM-red can be used to image protein sulfhydryl groups in live cells and in vivo. FM-red also could be used to measure of the redox states of thioredoxin (Trx)[1].
FM-red (10 μM; 30 min) is suitable for imaging protein thiols in HeLa cells[1].FM-red (20 μM; 48 h) has no significant cytotoxicity in HeLa cells[1].FM-red possesses a long emission wavelength (~655 nm) and fast response (~10 min) by binding to protein thiols[1].FM-red exhibits time- and concentration-dependent fluorescence response to bovine serum albumin (BSA)[1].
FM-red (10 μM; 30 min) could be used to image protein thiols in zebrafishes[1].
[1]. Hu G, et, al. Depletion of protein thiols and the accumulation of oxidized thioredoxin in Parkinsonism disclosed by a red-emitting and environment-sensitive probe. J Mater Chem B. 2019 Apr 28;7(16):2696-2702.
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