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Violuric acid 是一种在漆酶体系中使用的氧化还原介体。Violuric acid 测定法是一种确定漆酶在定向进化过程中不丧失高氧化还原电位的方法。
Violuric acid is a redox mediator used in the laccase system. The violuric acid assay is a method to ascertain that the high-redox potential of laccase is not lost during directed evolution[1][2].
参考文献:
[1]. Zhenyu Shi, et al. Enhanced Transformation of Emerging Contaminants by Permanganate in the Presence of Redox Mediators. Environ Sci Technol. 2020 Feb 4;54(3):1909-1919.
[2]. Isabel Pardo, et al. Colorimetric High-Throughput Screening Assays for the Directed Evolution of Fungal Laccases. Methods Mol Biol. 2018;1685:247-254.
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