An activator of chaperone-mediated autophagy
QX-77 is an activator of chaperone-mediated autophagy (CMA).1 It decreases mouse embryonic fibroblast (MEF) and Neuro2a cell death induced by paraquat or oleic acid . QX-77 (20 ?M) rescues defective trafficking and lysosomal localization of the CMA receptor LAMP2A in Ctns-/- MEFs and CTNS knockout human proximal tubule cells, models of the lysosomal storage disorder cystinosis.2
1.Cuervo, A.M., Gavathiotis, E., Xin, Q., et al.Retinoic acid receptor antagonists as chaperone-mediated autophagy modulators and uses thereof(2015) 2.Zhang, J., Johnson, J.L., He, J., et al.Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2AJ. Biol. Chem.292(25)10328-10346(2017)
Cell experiment: | Mouse embryo fibroblasts are preincubated with QX77 or vehicle (DMSO) 48 h before the addition of tert-butyl hydroperoxide. After an overnight incubation, cell viability is determined by a modified version of the MTT assay[1]. |
参考文献: [1]. Zhang J, et al. Cystinosin, the small GTPase Rab11, and the Rab7 effector RILP regulate intracellular trafficking of the chaperone-mediated autophagy receptor LAMP2A. J Biol Chem. 2017 Jun 23;292(25):10328-10346. | |
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