Iterative in situ click chemistry (IISCC)

迭代原位点化学

英文解释

Iterative in situ click chemistry (IISCC) is a robust general technology for development of high throughput, inexpensive protein detection agents. In IISCC, the target protein acts as a template and catalyst, and assembles its own ligand from modular blocks of peptides. This process of ligand discovery is iterated to add peptide arms to develop a multivalent ligand with increased affinity and selectivity. The peptide based protein capture agents (PCC) should ideally have the same degree of selectivity and specificity as a monoclonal antibody, along with improved chemical stability.

中文解释

迭代原位点化学 (IISCC) 是开发高通量、低成本蛋白质检测试剂的一种稳健的通用技术。在 IISCC 中，靶蛋白作为模板和催化剂，从多肽模块组装自己的配体。这个发现配体的过程是重复的，添加肽臂，以开发具有更高亲和力和选择性的多价配体。基于肽的蛋白质捕获剂 (PCC) 理想情况下应该具有与单克隆抗体相同的选择性和特异性，以及更好的化学稳定性。

参考文献

[1]. Dissertation. Developing Peptide Based Capture Agents for Diagnostics and Therapeutics. Developing Peptide Based Capture Agents for Diagnostics and Therapeutics. 2013.