Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis (SDS-PAGE)
发布时间:2023-03-22 22:08:39
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Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis (SDS-PAGE)
十二烷基硫酸钠聚丙烯酰胺凝胶电泳
英文解释
SDS-PAGE is an electrophoresis method that allows protein separation by massbetween 5 and 250 kDa.SDS-PAGE has the full name of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the?method is composed of gel preparation, sample preparation, electrophoresis,protein staining or western blotting?and analysis of the generated banding pattern.
中文解释
十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE) 是一种电泳方法,能够按质量分离在 5-250 kDa 之间的蛋白质。SDS-PAGE 全称十二烷基硫酸钠-聚丙烯酰胺凝胶电泳,该方法包括:凝胶制备、样品制备、电泳、蛋白染色或蛋白印迹法以及分析生成的条带图。
参考文献
[1]. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680-5.
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