WST-8
A chromogenic indicator for NADH
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包装价格促销价数量
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25mg￥432.00346.00- +
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50mg￥692.00554.00- +
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100mg￥1038.00831.00- +
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200mg￥1732.001386.00- +
- 货号: ajcx11028
- CAS: 193149-74-5
- 别名: 2-(2-甲氧基-4-硝基苯)-3-(4-硝基苯)-5-(2,4-二磺基苯)-2H-四氮唑单钠盐
- 分子式: C20H14N6NaO11S2+
- 分子量: 600.47
- 纯度: ＞98%
- 溶解度: DMSO: 1 mg/ml,PBS (pH 7.2): 5 mg/ml
- 储存: Store at -20°C,protect from light
- 库存: 现货
Background
WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.
The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line[1].
Reference：
[1]. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54.
