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Reagent used for tyramide signal amplification (TSA)
货号:ajcx11112
CAS:
分子式:C30H47N5O5S
分子量:589.79
溶解度:≥ 59mg/mL in DMSO, ≥ 14.1 mg/mL in EtOH with ultrasonic
纯度:98%
存储:Store at -20°C
库存:现货
Background:
Biotin-LC-LC-tyramide is used for tyramide signal amplification (TSA) which is a powerful, patented technology that significantly enhances both chromogenic and fluorescent signals. TSA is easily integrated into standard nonradioactive in situ hybridization (ISH) or IHC protocols.
TSA is an enzyme-mediated detection method that uses horseradish peroxidase (HRP) to catalyze the deposition of a fluorophore-labeled tyramide amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins.
Biotin-LC-LC-tyramide use horseradish peroxidase (HRP) to catalyze covalent deposition of biotin labels directly adjacent to the immobilized enzyme. The labeling reaction is quick (less than 10 minutes) and deposited labels can be detected with streptavidin conjugates for imaging in brightfield or fluorescence microscopy.
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