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  • Cy5 alkyne (non-sulfonated)
Cy5 alkyne (non-sulfonated)的可视化放大

Cy5 alkyne (non-sulfonated)

used for Click chemistry to label any molecule bearing azide group.

原价
¥3925-11737
价格
3140-9390
Cy5 alkyne (non-sulfonated)的二维码

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  • 货号: ajcx11310
  • CAS: N/A
  • 别名:
  • 分子式: C35H42ClN3O
  • 分子量: 556.18
  • 纯度: >98%
  • 溶解度: ≥ 55.6mg/mL in DMSO, ≥ 50 mg/mL in EtOH with ultrasonic
  • 储存: Store at -20°C
  • 库存: 现货

Background

Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Various substrates such as biomolecules, polymers, and solid surfaces which bearing azides can be used for the labeling.


In the azido-GalNAc samples, alkyne-Cy3 and alkyne-Cy5 specifically label azido-proteins. Furthermore, researches also indicate that the Cy5 and Cy3 probe-protein conjugates have non-overlapping fluorescence profiles and can be differentially detected within a single gel [1].

Reference:
[1] Burnham-Marusich, A.?R.; Plechaty, A.M.; Berninsone, P.M. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis, 2014, 35(18), 2621-2625.

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