Cy5 alkyne (non-sulfonated)

used for Click chemistry to label any molecule bearing azide group.

此产品仅用于科学研究，我们不为任何个人用途提供产品和服务

库存: 现货

可选规格

包装

价格

促销价

数量
5mg

￥3925.00

3140.00

- +
25mg

￥11737.00

9390.00

- +

已选 0 种 0 瓶

金额: ￥0.00

首页收藏

货号: ajcx11310
CAS: N/A
别名:
分子式: C35H42ClN3O
分子量: 556.18
纯度: >98%
溶解度: ≥ 55.6mg/mL in DMSO, ≥ 50 mg/mL in EtOH with ultrasonic
储存: Store at -20°C
库存: 现货

Background

Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Various substrates such as biomolecules, polymers, and solid surfaces which bearing azides can be used for the labeling.

In the azido-GalNAc samples, alkyne-Cy3 and alkyne-Cy5 specifically label azido-proteins. Furthermore, researches also indicate that the Cy5 and Cy3 probe-protein conjugates have non-overlapping fluorescence profiles and can be differentially detected within a single gel [1].

Reference:
[1] Burnham-Marusich, A.?R.; Plechaty, A.M.; Berninsone, P.M. Size-matched alkyne-conjugated cyanine fluorophores to identify differences in protein glycosylation. Electrophoresis, 2014, 35(18), 2621-2625.