- 首页
- HSP (Heat shock proteins)
- Photo-lysine hydrochloride
Photo-lysine hydrochloride
Photo-lysinehydrochloride，一种新的基于赖氨酸光反应性的氨基酸，它捕获结合赖氨酸翻译后修饰的蛋白质。
此产品仅用于科学研究，我们不为任何个人用途提供产品和服务
-
包装价格促销价数量
-
1mg￥7500.006000.00- +
-
5mg￥12825.0010260.00- +
-
10mg￥23075.0018460.00- +
-
20mg￥38475.0030780.00- +
- 货号: ajcx13326
- CAS: N/A
- 别名:
- 分子式: C6H14Cl2N4O2
- 分子量: 245.11
- 纯度: ＞98%
- 溶解度: Water : ≥ 40 mg/mL (163.19 mM)
- 储存: Store at -20°C
- 库存: 现货
Background
Photo-lysine hydrochloride, a new lysine-based photo-reactive amino acid, captures proteins that bind lysine post-translational modifications.
Photo-lysine is designed and synthesized by incorporating a photo-cross-linker (diazirine) into the side chain of natural lysine. Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including 'readers' and 'erasers' of histone modifications. Photo-lysine can be incorporated into MDH2 and mediate photo-cross-linking to fix protein-protein interactions in cells. UV irradiation of cells in the presence of photo-lysine induced robust cross-linking of HSP90β and HSP60. Photo-lysine has higher efficiency than photo-leucine for photo-cross-linking of the two chaperone proteins. Photo-lysine enables capture of the heterodimer of proteins Ku70 and Ku80 within a protein complex. Photo-lysine enables identification of histone- and chromatin-binding proteins[1].
[1]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2.
