A modifier of protein sulfhydryl groups
N-Ethylmaleimide is a modifier of sulfhydryl groups in proteins.1,2 It has commonly been used to inactivate cysteine-containing enzymes in vitro. N-Ethylmaleimide inhibits prolyl endopeptidase with an IC50 value of 6.3 ?M.3 It alkylates cysteine 519 (Cys519) in voltage-gated potassium channel 7.4 (Kv7.4) and activates Kv7.2 , Kv7.4, and Kv7.5, but not Kv7.3, in CHO cells expressing the human channels when used at a concentration of 50 ?M.4
1.Kawakita, M., and Yamashita, T.Reactive sulfhydryl groups of sarcoplasmic reticulum ATPase. III. Identification of cysteine residues whose modification with N-ethylmaleimide leads to loss of the Ca2+-transporting activityJ. Biochem.102(1)103-109(1987) 2.Fatania, H.R., al-Nassar, K.E., and Thomas, N.Chemical modification of rat liver cytosolic NADP+-linked isocitrate dehydrogenase by N-ethylmaleimide. Evidence for essential sulphydryl groupsFEBS Lett.322(3)245-248(1993) 3.Moriyama, A., Nakanishi, M., and Sasaki, M.Porcine muscle prolyl endopeptidase and its endogenous substratesJ. Biochem.104(1)112-117(1988) 4.Li, Y., Gamper, N., and Shapiro, M.S.Single-channel analysis of KCNQ K+ channels reveals the mechanism of augmentation by a cysteine-modifying reagentJ. Neurosci.24(22)5079-5090(2004)
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