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- ALK (Anaplastic lymphoma kinase)
- N-Ethylmaleimide
N-Ethylmaleimide
A modifier of protein sulfhydryl groups
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包装价格促销价数量
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10mg￥425.00340.00- +
- 货号: ajcx13604
- CAS: 128-53-0
- 别名: N-乙基马来酰亚胺; NEM
- 分子式: C6H7NO2
- 分子量: 125.13
- 纯度: ＞98%
- 溶解度: DMSO : 50 mg/mL (399.58 mM) Water : 50 mg/mL (399.58 mM), Ethanol : 12.5 mg/mL (99.90 mM)
- 储存: Store at 4°C, sealed storage, away from moisture and light
- 库存: 现货
Background
N-Ethylmaleimide is a modifier of sulfhydryl groups in proteins.1,2 It has commonly been used to inactivate cysteine-containing enzymes in vitro. N-Ethylmaleimide inhibits prolyl endopeptidase with an IC50 value of 6.3 ?M.3 It alkylates cysteine 519 (Cys519) in voltage-gated potassium channel 7.4 (Kv7.4) and activates Kv7.2 , Kv7.4, and Kv7.5, but not Kv7.3, in CHO cells expressing the human channels when used at a concentration of 50 ?M.4
1.Kawakita, M., and Yamashita, T.Reactive sulfhydryl groups of sarcoplasmic reticulum ATPase. III. Identification of cysteine residues whose modification with N-ethylmaleimide leads to loss of the Ca2+-transporting activityJ. Biochem.102(1)103-109(1987) 2.Fatania, H.R., al-Nassar, K.E., and Thomas, N.Chemical modification of rat liver cytosolic NADP+-linked isocitrate dehydrogenase by N-ethylmaleimide. Evidence for essential sulphydryl groupsFEBS Lett.322(3)245-248(1993) 3.Moriyama, A., Nakanishi, M., and Sasaki, M.Porcine muscle prolyl endopeptidase and its endogenous substratesJ. Biochem.104(1)112-117(1988) 4.Li, Y., Gamper, N., and Shapiro, M.S.Single-channel analysis of KCNQ K+ channels reveals the mechanism of augmentation by a cysteine-modifying reagentJ. Neurosci.24(22)5079-5090(2004)
