A broad spectrum kinase inhibitor used in proteomics
CTX-0294885 is a broad spectrum kinase inhibitor that inhibited FAK, FLT3, JAK2, JAK3, Src, Aurora kinase A, and VEGF receptor 3 (IC50s = 4, 1, 3, 28, 2, 18, and 3 nM, respectively) in an initial screen.1 In an affinity purification experiment, CTX-0294885 beads bound to kinases from every major group and captured all members of the AKT family of proteins. In a large-scale kinome profiling experiment, it captured 235 kinases from MDA-MB-231 breast cancer cells.
1.Zhang, L., Holmes, I.P., Hochgr?fe, F., et al.Characterization of the novel broad-spectrum kinase inhibitor CTx-0294885 as an affinity reagent for mass spectrometry-based kinome profilingJ. Proteome Res.12(7)3104-3116(2013)
动态评分
0.0