A protein cross-linking reagent
SMCC is a heterobifunctional cross-linking reagent that contains an amine-reactive N-hydroxysuccinimide ester and a sulfhydryl-reactive maleimide group that can be used in click chemistry reactions in the conjugation of proteins.1,2,3 It has been used to cross-link the anticancer agent DM1 to an IgG1 monoclonal antibody to form the antibody-drug conjugate DM1-SMCC .4 Immunization of mice with SMCC-coupled keyhole limpet hemocyanin (KLH) antigen and syngeneic splenocytes increases the percentage of proliferating T cells in isolated splenocytes from recipient mice compared to mice immunized with KLH and the adjuvant LPS.3
1.Bieniarz, C., Husain, M., Barnes, G., et al.Extended length heterobifunctional coupling agents for protein conjugationsBioconjug. Chem.7(1)88-95(1996) 2.Hermanson, G.T.The reactions of bioconjugationBioconjugate Techniques(2013) 3.Guo, Y., Werbel, T., Wan, S., et al.Potent antigen-specific immune response induced by infusion of spleen cells coupled with succinimidyl-4-(N-maleimidomethyl cyclohexane)-1-carboxylate (SMCC) conjugated antigensInt. Immunopharmacol.31158-168(2019) 4.Luo, Q., Chung, H.H., Borths, C., et al.Structural characterization of a monoclonal antibody-maytansinoid immunoconjugateAnal. Chem.88(1)695-702(2016)
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