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A fluorogenic peptide substrate
Bz-FVR-AMC is a fluorogenic peptide substrate for bovine cathepsins S and H, Xenopus endopeptidase XSCEP1, and esterase E-II from B. gabonica venom, among others. Upon enzymatic cleavage, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify enzyme activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.
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