A fluorescent protein labeling agent
7-Amino-4-methyl-3-coumarinylacetic acid (AMCA) is fluorescent protein labeling agent.1 It contains an N-hydroxysuccinimide ester that reacts with lysine residues to form photostable amide links. Upon activation with UV light, AMCA displays emission maxima of 400-460 nm. It has commonly been used in multiplex immunophenotyping applications.2,3,4
|1. Khalfan, H., Abuknesha, R., Rand-Weaver, M., et al. Aminomethyl coumarin acetic acid: A new fluorescent labelling agent for proteins. Histochem. J. 18(9), 497-499 (1986).|2. Ferri, G.-L., Gaudio, R.M., Castello, I.F., et al. Quadruple immunofluorescence: A direct visualization method. J. Histochem. Cytochem. 45(2), 155-158 (1997).|3. Nederlof, P.M., Robinson, D., Abuknesha, R., et al. Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences. Cytometry 10(1), 20-27 (1989).|4. Gothot, A., Grosdent, J.-C., and Paulus, J.-M. A strategy for multiple immunophenotyping by image cytometry: Model studies using latex microbeads labeled with seven streptavidin-bound fluorochromes. Cytometry 24(3), 214-225 (1996).
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