Background
15-deoxy-δ12,14-Prostaglandin J2 is one of the cyclopentenone PGs, which have documented metabolic (peroxisome proliferator-activated receptor γ-activating), antimitotic, and antiproliferative effects.1,2,3 The activity of the compounds in this class, which includes PGs in both the A- and J-series, may result from changes in gene expression and the interaction with non-classical (i.e., non-G protein-coupled receptor) pathways. 15-deoxy-δ12,14-PGJ2-biotin is an affinity probe which allows 15-deoxy-δ12,14-PGJ2 to be detected through an interaction with the biotin ligand. 15-deoxy-δ12,14-PGJ2-biotin was designed to allow 15-deoxy-δ12,14-PGJ2 to be detected in complexes with nuclear receptors and/or nucleic acid or protein binding partners. It is thus a tool to be used in the general elucidation of the mechanism of action of the cyclopentenone PGs.
1.Krakoff, L.R., Vlachakis, N., Mendlowitz, M., et al.Differential effect of prostaglandin A1 in hypertensive patients with low, normal and high reninClinical Science and Molecular Medicine48311s-313s(1975)
2.Kikuchi, Y., Kita, T., Hirata, J., et al.Preclinical studies of antitumor prostaglandins by using human ovarian cancer cellsCancer Metastasis Rev.13(3-4)309-315(1994)
3.Mueller, E., Drori, S., Aiyer, A., et al.Genetic analysis of adipogenesis through peroxisome proliferator-activated receptor γ isoformsThe Journal of Biological Chemisty277(44)41925-41930(2002)
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