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A fluorescent substrate for NADH:ubiquinone oxidoreductases
Coumarin-quinone conjugate is a fluorescent substrate for NADH:ubiquinone oxidoreductases.1 It is comprised of a coumarin fluorophore conjugated to a ubiquinone analog that can be reduced by NADH:ubiquinone oxidoreductases. It has been used to measure the kinetic parameters of apoptosis-inducing factor mitochondria-associated 2/ferroptosis suppressor protein 1 (AIFM2/FSP1).
1.Doll, S., Freitas, F.P., Shah, R., et al.FSP1 is a glutathione-independent ferroptosis suppressorNature575(7784)693-698(2019)
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