A neuropeptide with diverse biological activities
Mca-YVADAP-K(Dnp)-OH is a substrate for caspase-1 and angiotensin-converting enzyme 2 (ACE2).1,2 Upon cleavage by caspase-1 or ACE2, 7-methoxycoumarin-4-acetyl (Mca) is released and its fluorescence can be used to quantify enzyme activity. Mca displays excitation/emission maxima of 328/420 nm, respectively.
1.Vickers, C., Hales, P., Kaushik, V., et al.Hydrolysis of biological peptides by human angiotensin-converting enzyme-related carboxypeptidaseJ. Biol. Chem.277(17)14838-14843(2002) 2.Enari, M., Talanian, R.V., Wong, W.W., et al.Sequential activation of ICE-like and CPP32-like proteases during Fas-mediated apoptosisNature380(6576)723-726(1996)
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