Background
The phosphatidylinositols (PtdIns) phosphates represent a small precentage of total membrane phospholipids. However, they play a critical role in the generation and transmission of cellular signals.1,2 PtdIns-(4,5)-P2 can be phosphorylated by phosphatidylinositol (PI)-3-kinase to make PtdIns-(3,4,5)-P3, which initiates an intricate signaling cascade that has been implicated in cancer.3 PtdIns-(3,4,5)-P3 fluorescein is a fluorescent probe for any protein with a high affinity binding interaction with inositol-(3,4,5)-triphosphate phospholipids, such as phosphatidylinositol 3-kinase, PTEN, or PH-domain-containing proteins.
1.Exton, J.H.Regulation of phosphoinositide phospholipases by hormones, neurotransmitters, and other agonists linked to G proteinsAnnu. Rev. Pharmacol. Toxicol.36481-509(1996)
2.Majerus, P.W.Inositol phosphate biochemistryAnnu. Rev. Biochem.61225-250(1992)
3.Vivanco, I., and Sawyers, C.L.The phosphatidylinositol 3-kinase-AKT pathway in human cancerNature Reviews.Cancer2489-501(2002)
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