An internal standard for the quantification of hyocholic acid
Hyocholic acid-d4 is intended for use as an internal standard for the quantification of hyocholic acid by GC- or LC-MS. Hyocholic acid is a primary bile acid in pigs and other mammals.1 It has also been found in urine samples from patients with cholestasis.2 Hyocholic acid is converted by gut microflora primarily to taurohyocholate and, to a lesser extent, taurocholic acid and tauro-β-muricholic acid in mice.3 Hyocholic acid has low toxicity against human hepatoma HepG2 cells.3,4
1.Lundell, K., and Wikvall, K.Species-specific and age-dependent bile acid composition: Aspects on CYP8B and CYP4A subfamilies in bile acid biosynthesisCurr. Drug Metab.9(4)323-331(2008) 2.van Berge Henegouwen, G.P., Brandt, K.H., Eyssen, H., et al.Sulphated and unsulphated bile acids in serum, bile, and urine of patients with cholestasisGut17(11)861-869(1976) 3.Wang, D.Q.-H., Tazuma, S., Cohen, D.E., et al.Feeding natural hydrophilic bile acids inhibits intestinal cholesterol absorption: Studies in the gallstone-susceptible mouseAm. J. Physiol Gastrointest. Liver Physiol.285(3)G494-G502(2003) 4.Perreault, M., Bialek, A., Trottier, J., et al.Role of glucuronidation for hepatic detoxification and urinary elimination of toxic bile acids during biliary obstructionPLoS One8(11)e80994(2013)
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