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- ALK (Anaplastic lymphoma kinase)
- 3-Indoxyl Phosphate (sodium salt)
3-Indoxyl Phosphate (sodium salt)
A chromogenic and electrochemical substrate for alkaline phosphatase
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包装价格促销价数量
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250mg￥1837.001470.00- +
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1g￥5100.004080.00- +
- 货号: ajcx25220
- CAS: 3318-43-2
- 别名: 3-吲哚酰磷酸酯二钠盐
- 分子式: C8H6NO4P·2Na
- 分子量: 257.1
- 纯度: ＞98%
- 溶解度: PBS (pH 7.2): 10 mg/ml
- 储存: -20°C
- 库存: 现货
Background
3-Indoxyl phosphate is a chromogenic and electrochemical substrate for alkaline phosphatase.1,2 Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity.1 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs.
1.Tsou, K.C., and Su, H.C.F.A new colorimetric method for the determination of alkaline phosphatase with indoxyl phosphateAnal. Biochem.11(1)54-64(1965) 2.Fanjul-Bolado, P., González-García, M.B., and Costa-García, A.Detection of leucoindigo in alkaline phosphatase and peroxidase based assays using 3-indoxyl phosphate as substrateAnal. Chim. Acta534(2)231-238(2005)
