A fluorescent derivatization reagent
1,3-Benzodioxole-5,6-diamine is a fluorescent derivatization reagent.1,2,3 It has been commonly used for the derivatization of α-keto, neuraminic, and sialic acids for detection by fluorometric HPLC.
1.Hara, S., Yamaguchi, M., Takemori, Y., et al.Highly sensitive determination of N-acetyl- and N-glycolylneuraminic acids in human serum and urine and rat serum by reversed-phase liquid chromatography with fluorescence detectionJ. Chromatogr.377111-119(1986) 2.Hayakawa, K., De Felice, C., Watanabe, T., et al.Determination of free N-acetylneuraminic acid in human body fluids by high-performance liquid chromatography with fluorimetric detectionJ. Chromatogr.620(1)25-31(1993) 3.Martín, M.J., Vázquez, E., and Rueda, R.Application of a sensitive fluorometric HPLC assay to determine the sialic acid content of infant formulasAnal. Bioanal. Chem.387(8)2943-2949(2007)
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