High affinity fluorescently tagged aptamer for EpCam
High affinity fluorescently tagged aptamer for EpCam (Kd = 39.42 nM in HT29 cells). Efficiently internalized upon binding to EpCAM. Exhibits rapid penetration of tumorspheres in vitro, as well as superior penetration, accumulation and retention in tumor xenografts in vivo than an antibody to the same target. For optimal binding aptamers require refolding into their tertiary structure prior to use. Please refer to the protocol for information regarding refolding and aptamer use in unfixed cell imaging and flow cytometry.
Shigdar et al (2011) RNA aptamer against a cancer stem cell marker epithelial cell adhesion molecule. Cancer Sci. 102 991 PMID:21281402 |Shigdar et al (2013) The use of sensitive chemical antibodies for diagnosis: detection of low levels of EpCAM in breast cancer. PLoS One. 8 e57613 PMID:23460885 |Xiang et al (2015) Superior performance of aptamer in tumor penetration over antibody: implication of aptamer-based theranostics in solid tumors. Theranostics. 5 1083 PMID:26199647
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