花青 3 Tyramide (Tyramide-Cy3) 是一种橙色荧光染料，被用作辣根过氧化物酶 (HRP) 催化沉积的报告荧光底物，这是免疫测定和核酸原位杂交中的信号放大技术。
Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, ISH, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.
Schmidt et al (1997) Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide. J.Histochem.Cytochem. 45 365 PMID:9071318 |van Gijlswijk et al (1997) Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 45 375 PMID:9071319 |Hopman et al (1998) Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 46 771 PMID:9603790 |Schriml et al (1999) Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 27 608 PMID:10489619 |Zhao et al (2013) Characterization of GABAergic neurons in the mouse lateral septum: a double fluorescence in situ hybridization and immunohistochemical study using tyramide signal amplification. PLoS One 8 e73750 PMID:23967349
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