TAMRA phosphoramidite, 5-isomer

This phosphoramidite isused for synthesis ofoligonucleotides 5'-labeled with TAMRA

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库存: 现货

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数量
250mg

￥5875.00

4700.00

- +
1g

￥14787.00

11830.00

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货号: ajcx28876
CAS: N/A
别名:
分子式: C40H50N5O6P
分子量: 727.83
纯度: >98%
溶解度: good in acetonitrile
储存: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
库存: 现货

Background

This phosphoramidite is?used for synthesis of?oligonucleotides 5'-labeled with TAMRA.

TAMRA(carboxytetramethylrhodamine) is?a?xanthene dye from the rhodamine family with emission in?the orangespectrum range (maximum at?563?nm). This fluorophore is?traditionally used as?a?FRET-acceptor(and a?quencher) in?a?pair with fluorescein (FAM) due to?significant overlapping of?theirspectra. Thus, this phosphoramidite is?convenient for the synthesis of?dual-labeled probes TaqMan, whichcontain 5'-terminal TAMRA and FAM in?the middle of?the sequence or?at?the 3'-end(using Fluorescein dT?Phosphoramidite and FAMCPG, respectively).

TAMRA 5'-labeled oligonucleotides are commonly used for quantitative PCRand fragment analysis (for example, for microsatellite marker analysis) because the equipment available has a?detectionchannel for TAMRA frequently.

The TAMRA dye is?not stable in?the presence of?ammonium andsterically non-hindered primary amines, so?it?is?strongly recommended to?follow specifiedconditions for labeled oligonucleotide deprotection.

Usage

Coupling: 7.5?min.

Deprotection: tret-buthylamine : methanol : water 1 : 1 : 3?(v/v/v) (?TAMRA cocktail?) for 6?hours at?60?°C, then cool down to?room temperature.

Due to?complete and irreversible degradation of?the TAMRA dye, do?NOT use aqueous ammonium and AMA for deprotecting a?modified oligonucleotide from the solid-phase support.