R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer
R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.
R6G (rhodamine?6G) is?a?xanthene dye of?the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to?those of?fluorescein, the absorption (518?nm) and emission (542?nm) maxima of?6-R6G are shifted into the long-wave region.
R6G can be?used as?a?FRET acceptor in?DNA-sequencing and in?short tandem repeat (STR) analysis. The dye is?commonly used for producing TaqMan probes for quantitative PCR. In?comparison with HEX and JOE dyes, R6G in?combination with BHQ1 quencher has stronger quenching in?molecular beacon probes, significantly reducing background fluorescence in?PCR.
Usage:Coupling: 10?minutes.
Deprotection: solution of?tert-Butylamine in?water (1:3?v/v) overnight at?55°С.
Aqueous ammonia and AMA (solution of?30% ammonium hydroxide/40% aqueous methylamine 1:1?v/v) must NOT be?used for deprotection of?the modified oligonucleotide from the solid-phase support because of?complete and irreversible R6G degradation.
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