Sulfo-Cyanine5is afar-red fluorophore widely used for biomolecule labeling, with excitation maximum at646nm and emission maximum at662nm
Sulfo-Cyanine5?is a?far-red fluorophore widely used for biomolecule labeling, with excitation maximum at?646?nm and emission maximum at?662?nm. Far-red fluorescent tags with excitation above 600?nm and emission further than 650?nm are valuable for imaging techniques because of?the lower background autofluorescence at?these wavelengths. Besides, far-red fluorescent labels can be?imaged simultaneously with near-red, orange, green, and blue tags, which is?advantageous for multicolor imaging.
Biotin, or?the water-soluble vitamin?H, is?well known not only for playing essential roles in?various crucial metabolic cellular reactions but also for its extremely high affinity for avidin, a?glycoprotein of?egg white. Avidin, streptavidin (bacterial analogue of?avidin), and neutravidin (deglycosylated avidin) bind non-cooperatively to?biotin with high affinity. Thus, sulfo-Cyanine5 biotin conjugate can be?used for detecting and quantifying biotin binding sites of?avidin, streptavidin, and neutravidin in?samples of?different origin.
Sulfo-Cyanine5 biotin conjugate is?a?water-soluble reagent and its fluorescence is?pH?independent from pH?4?to?pH?10. A?flexible PEG3 linker between biotin moiety and fluorescent tag provides binding to?avidin, streptavidin, or?neutravidin without steric troubles.
The major applications of?sulfo-Cyanine5 biotin conjugate include imaging (e.g. primary and secondary antibody labeling for Western Blotting, immunoassay, cyto- and histochemistry, flow cytometry), affinity and dissociation constant measuring, streptavidin-based sensors, etc.
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