HBC620

HBC620 是 HBC 模拟。

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库存: 现货

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5mg

￥3262.00

2610.00

- +
10mg

￥5175.00

4140.00

- +
50mg

￥14962.00

11970.00

- +
100mg

￥23512.00

18810.00

- +

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货号: ajcx29384
CAS: 2530162-07-1
别名:
分子式: C19H15N3OS2
分子量: 365.47
纯度: >98%
溶解度: DMSO:16.67 mg/mL (45.61 mM)
储存: Store at -20°C,unstable in solution, ready to use.
库存: 现货

Background

HBC620 is an analog of HBC, which is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor. HBC is nonfluorescent in solution, but strongly fluoresces upon constraining intramolecular motion. HBC-Pepper complex can be used to visualize RNA dynamics in live cells^[1].

HBC's fluorescence can be enhanced more than 3000-fold in vitro by Pepper. Mammalian cells expressing Pepper exhibited robust greenyellowish fluorescence on incubation with 1μM HBC^[1].

参考文献:
[1].Chen X, Zhang D, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293.

HBC620 是 HBC 的类似物，它是一种类似 GFP 荧光团的合成染料，具有结构刚性的电子受体和强电子供体。 HBC 在溶液中不发荧光，但在限制分子内运动时会发出强烈的荧光。 HBC-Pepper 复合物可用于可视化活细胞中的 RNA 动力学^[1]。

HBC 的荧光在体外可通过 Pepper 增强 3000 多倍。表达 Pepper 的哺乳动物细胞在与 1μM HBC 孵育时表现出强烈的黄绿色荧光^[1]。

Protocol

Cell experiment [1]:
Cell lines	293T/17 cells
Preparation Method	293T/17 cells were transfected with the corresponding plasmids. After 30h, the cells were digested and resuspended in the 4% FBS/1× PBS solution containing 0.2μM HBC and 5mM MgSO₄. The fluorescence was analyzed using a CytoFLEX-S flow cytometer.
Reaction Conditions	0.2μM HBC
Applications	When cells co-expressing Pepper-tagged centromere-targeting sgRNA and dCas9-GFP were incubated with the HBC620 fluorophore, numerous (~40) nuclear foci of Pepper620 fluorescence nearly perfectly co-localized with GFP fluorescence.
参考文献: [1]. Chen X, Zhang D, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293..