A free radical generator
Phenazine is a free radical generator.1 It has been used as an electron transfer reactant in cell viability assays.2 Phenazine (10 ?M) induces ssDNA break formation in the presence of the reducing agent NADPH in a cell-free plasmid cleavage assay when used at a concentration of 10 ?M. It induces oxidative DNA damage in an alkaline comet assay and apoptosis in A375 melanoma cells when used at a concentration of 10 ?M. Phenazine (20 nM) oxidizes cysteine-containing proteins in HepG2 cells.1
1.Lin, C.-Y., Hu, C.-T., Cheng, C.-C., et al.Oxidation of heat shock protein 60 and protein disulfide isomerase activates ERK and migration of human hepatocellular carcinoma HepG2Oncotarget7(10)11067-11082(2016) 2.Hua, A.B., Justiniano, R., Perer, J., et al.Repurposing the electron transfer reactant phenazine methosulfate (PMS) for the apoptotic elimination of malignant melanoma cells through induction of lethal oxidative and mitochondriotoxic stressCancers (Basel)11(5)E590(2019)
动态评分
0.0