Naph-EA-mal(Thiol-green1)是一种快速检测和超快turn-on型硫醇(thiol)荧光探针，可用于蛋白标记和生物成像。Naph-EA-mal(Thiol-green1)可用于检测活细胞中的硫醇，标记蛋白质硫醇，定量细胞裂解液中总硫醇的浓度，并确定固定细胞中可逆的蛋白质硫醇氧化。Ex:488nM;Em:540nM。
Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells[1]. Ex: 488 nM; Em: 540 nM.
Imaging thiols in living cells[1]Hep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 µM;5 mins), and strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 µM), a weaker fluorescence is observed in cells[1].Monitoring thiol-disulfide levels in fixed cellsDue to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 µM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed[1].The Hep G2 cells, oxidized by diamide (200 µM; 30 min), are fixed by 70% EtOH and the remaining free thiols are blocked by NEM (100 µM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 µM). The green fluorescent signal is detected again[1]
[1]. Jinyu Sun, et al. An ultrafast turn-on thiol probe for protein labeling and bioimaging. Analyst
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