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  • EDANS
EDANS的可视化放大

EDANS

EDANS(1,5-EDANS)是一种新型的淬灭荧光底物(fluorogenicsubstrate),用于通过共振能量转移分析逆转录病毒蛋白酶。

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¥550-550
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440-440
EDANS的二维码

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  • 货号: ajcx32404
  • CAS: 50402-56-7
  • 别名: 5-(2-氨基乙氨基)-1-萘磺酸,1,5-EDANS
  • 分子式: C12H14N2O3S
  • 分子量: 266.32
  • 纯度: >98%
  • 溶解度: DMSO: 13.89 mg/mL (52.16 mM)
  • 储存: 4°C, protect from light, stored under nitrogen
  • 库存: 现货

Background

EDANS (1,5-EDANS) is a novel and quenched fluorogenic substrate for assaying retroviral protease by resonance energy transfer (RET)[1].


The peptide sequence of EDANS is derived from a natural processing site for HIV-1 PR[1].Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that was linearly related to the extent of substrate hydrolysis[1].


[1]. E D Matayoshi, et al. Novel fluorogenic substrates for assaying retroviral proteases by resonance energy transfer. Science. 1990 Feb 23;247(4945):954-8.

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