A fluorescent substrate of β-glucosidase and β-glucocerebrosidase
4-Methylumbelliferyl-β-D-Glucopyranoside (4-MUG) is a fluorogenic substrate of β-glucosidase and β-glucocerebrosidase (also known as glucosylceramidase). In addition to its use in characterizing novel β-glucosidases, 4-MUG is used in assays to evaluate deficiency in β- glucocerebrosidase activity related to Gaucher disease.1,2,3 Hydrolysis of 4-MUG releases the fluorescent product 4-MU, which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.4
参考文献:
1. Hernández-Guzmán, A., Flores-Martínez, A., Ponce-Noyola, P., et al. Purification and characterization of an extracellular β-glucosidase from Sporothrix schenckii FEBS Open Bio. 6(11), 1067-1077 (2016).
2. Kwapiszewski, R., Skolimowski, M., Zió?kowska, K., et al. A microfluidic device with fluorimetric detection for intracellular components analysis Biomed Microdevices 13(3), 431-440 (2011).
3. Shanmuganathan, M.K., and Britz-McKibbin, P. Inhibitor screening of pharmacological chaperones for lysosomal β-glucocerebrosidase by capillary electrophoresis Anal. Bioanal. Chem. 399(8), 2843-2853 (2011).
4. Zhi, H., Wang, J., Wang, S., et al. Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsule J. Spectrosc. 1(1), 147128 (2013).
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