An orally active inhibitor of p38α and p38β
IC50: 1 and 11 μM for p38α and p38β, respectively
RWJ 67657 is an orally active inhibitor of the MAP kinases p38α and p38β.
Tumor necrosis factor-alpha (TNF-alpha), a cytokine secreted by activated monocytes/macrophages and T lymphocytes, is implicated in several disease states, such as inflammatory bowel disease, rheumatoid arthritis, septic shock, as well as osteoporosis. The monocyte/macrophage production of TNF-alpha is dependent on the mitogen-activated protein kinase p38.
In vitro: RWJ 67657 could inhibit the release of TNF-alpha by lipopolysaccharide-treated human peripheral blood mononuclear cells, as well as the release of TNF-alpha from peripheral blood mononuclear cells treated with the superantigen staphylococcal enterotoxin B. RWJ 67657 was about 10-fold more potent than the literature standard p38 kinase inhibitor SB 203580 in all tested p38 dependent in-vitro systems tested. Moreover, RWJ 67657 was able to inhibit the enzymatic activity of recombinant p38alpha and beta, but not gamma or delta, and showed no significant activity against various other enzymes. In contrast, SB 203580 could significantly inhibit the tyrosine kinases p56 lck and c-src. In addition, RWJ 67657 did not inhibit T cell production of interleukin-2 or interferon-gamma and did not inhibit T cell proliferation in response to mitogens [1].
In vivo: Animal study found that RWJ 67657 could inhibit TNF-alpha production in lipopolysaccharide-injected mice (87% inhibition at 50 mg/kg) and in rats (91% inhibition at 25 mg/kg) after oral administration [1].
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Wadsworth, S.?A.,Cavender, D.E.,Beers, S.A., et al. RWJ 67657, a potent, orally active inhibitor of p38 mitogen-activated protein kinase. Journal of Pharmacology and Experimental Therapeutics 291(2), 680-687 (1999).
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