A-71623 是一种基于 CCK-4 的肽，是一种有效且高度选择性的 CCK-A 完全激动剂。
A-71623 is a selective agonist of CCKA receptor with an IC50 value of 3.7 nM in guinea pig pancreas [1, 2].
CCKA receptors belong to a subtype of cholecystokinin (CCK) receptors in the brain. CCK is a type of neuropeptide present throughout the central nervous system. CCK can act as a neurotransmitter in both normal and abnormal brain. CCK receptors exist in two forms in the brain. Another subtype of CCK receptors is CCKB subtype [1].
In NCI-H345 cells possessing CCKB/gastrin receptors, A-71623 was weak and behaved as a partial agonist in calcium studies [2]. A-71623 had very low affinity to CCK binding sites in C6 cells with an IC50 value of 1236 ± 81 nM [3]. It is hard to find the CCKA response result of the application of A-71623 in cells.
In radioligand binding assays, A-71623 showed IC50 values of 3.7 nM for CCKA in guinea pig pancreas and 4500 nM for CCKB in cerebral cortex. Data showed that A-71623 was an agonist in stimulating the release of pancreatic amylase, and this stimulatory effect was potently inhibited by L-364,718, a CCKA antagonist. Data showed that A-71623 acted as a full agonist in stimulating the breakdown of phosphoinositide in pancreas. In the ileum, A-71623 was also a potent agonist in stimulating CCKA receptors. In guinea pig gastric glands, the affinity of A-71623 for the CCK-B/gastrin receptor was 11 μM. This result demonstrated that A-71623 should be a potent and selective agonist at CCKA receptors [2].
参考文献:
[1].? Gracey DJ, Bell R, King DJ. Differential effects of the CCKA receptor ligands PD-140,548 and A-71623 on latent inhibition in the rat. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 2002, 26(3): 497-504.
[2].? Lin CW, Shiosaki K, Miller TR, et al. Characterization of two novel cholecystokinin tetrapeptide (30-33) analogues, A-71623 and A-70874, that exhibit high potency and selectivity for cholecystokinin-A receptors. Molecular pharmacology, 1991, 39(3): 346-351.
[3].? Kaufmann R, Lindschau C, Scho T, et al. Type B cholecystokinin receptors on rat glioma C6 cells. Binding studies and measurement of intracellular calcium mobilization. Brain research, 1994, 639(1): 109-114.
动态评分
0.0