Vitamin E derivative with antioxidant properties
Trolox is a vitamin E analogue and is a powerful antioxidant.
Trolox has shown to protect mammalian cells from oxidative damage. Trolox is effective in preventing myocyte necrosis in cell culture studies and in a canine model of two hours of left anterior descending coronary artery (LAD) occlusion followed by four hours of reperfusion[1]. Trolox could prevent oxidative stress-induced apoptosis in thymocytes. Pre- or posttreatment of cells with Trolox reduced H2O2-induced DNA fragmentation to control levels and below. [2]. Trolox is a hydrophilic analogue of alpha-tocopherol and reported to scavenge peroxyl radicals better than vitamin E in sodium dodecyl sulfate micelles and in liposomes. Trolox prolongs substantially the survival of human ventricular myocytes and hepatocyte against oxyradicals generated with xanthine oxidase plus hypoxanthine, and prevented lysis of red cells exposed to an azo-initiator[3].
Trolox是维生素E的类似物，是一种强效的抗氧化剂。
Trolox已经被证明可以保护哺乳动物细胞免受氧化损伤。在细胞培养研究和两个小时的左前降支冠状动脉（LAD）阻塞后四小时再灌注的犬模型中，Trolox能够有效预防肌细胞坏死[1]。Trolox可以预防氧化应激诱导的胸腺细胞凋亡。细胞的预处理或后处理使用Trolox将H2O2诱导的DNA断裂降至控制水平以下[2]。Trolox是α-生育酚的亲水性类似物，据报道在十二烷基硫酸钠胶束和脂质体中可以更好地清除过氧自由基。Trolox显著延长了人类室壁肌细胞和肝细胞在黄嘌呤氧化酶加上次黄嘌呤产生的氧自由基作用下的存活时间，并预防了暴露于偶氮引发剂下的红细胞的溶解[3]。
参考文献:
[1]. Mickle DA, et al. Myocardial salvage with trolox and ascorbic acid for an acute evolving infarction. Ann Thorac Surg. 1989 Apr;47(4):553-7.
[2]. Forrest VJ, et al. Oxidative stress-induced apoptosis prevented by Trolox. Free Radic Biol Med. 1994 Jun;16(6):675-84.
[3]. Wu TW, et al. The cytoprotective effect of Trolox demonstrated with three types of human cells. Biochem Cell Biol. 1990 Oct;68(10):1189-94.
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Cell experiment: |
Thymocytes are treated with Trolox (10 mM) before, during, or after 10 min exposure to 10 μM H2O2. Trolox incubations that are conducted before or after H2O2 exposures are performed in TCM. Trolox treatments that are administered concurrently with H2O2 are performed in PBS with ferrous sulfate[1]. |
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参考文献: [1]. Mickle DA, et al. Myocardial salvage with trolox and ascorbic acid for an acute evolving infarction. Ann Thorac Surg. 1989 Apr;47(4):553-7. |
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