coumarin-SAHA

A fluorescent probe for HDAC

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1mg

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460.00

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5mg

￥1487.00

1190.00

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10mg

￥2237.00

1790.00

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25mg

￥4162.00

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货号: ajci70878
CAS: 1260635-77-5
别名: coumarin-Suberoylanilide Hydroxamic Acid
分子式: C18H22N2O5
分子量: 346.4
纯度: >98%
溶解度: 5mg/mL in DMSO, 5mg/mL in DMF
储存: Store at -20°C
库存: 现货

Background

Suberoylanilide hydroxamic acid (SAHA) is a class I and class II histone deacetylase (HDAC) inhibitor that binds directly to the catalytic site of the enzyme thereby blocking substrate access. [1]? coumarin-Suberoylanilide hydroxamic acid (c-SAHA) is a SAHA derivative where the anilino "cap" group is replaced by 7-amino-4-methylcoumarin to produce a fluorescent probe that competitively binds HDAC.? [2] The fluorescence excitation and emission maxima of free c-SAHA is 325 and 400 nm, respectively and is quenched by 50% when bound to HDAC8. [2] This probe can be used to determine binding affinities and dissociation off-rates of HDAC enzyme-inhibitor complexes and is well-suited for high-throughput screening.

Reference:
[1]. Marks, P.A., and Breslow, R. Dimethyl sulfoxide to vorinostat: Development of this histone deacetylase inhibitor as an anticancer drug. Nat. Biotechnol. 25(1), 84-90 (2007).
[2]. Singh, R.K., Mandal, T., Balasubramanian, N., et al. Coumarin-suberoylanilide hydroxamic acid as a fluorescent probe for determining binding affinities and off-rates of histone deacetylase inhibitors. Anal. Biochem. 408(2), 309-315 (2011).