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A fluorescent biotin derivative
Fluorescein biotin (FITC biotin) is a fluorescent biotin derivative and avidin and streptavidin ligand that contains a FITC fluorophore which displays excitation/emission maxima of 485/535 nm, respectively. FITC fluorescence is quenched upon binding to avidin or streptavidin and decreases in fluorescence intensity have been used to quantify biotin binding stoichiometry.
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