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  • H100
H100的可视化放大

H100

H100是一种氯离子运输(Cl-transport)抑制剂,可以部分抑制氯离子载体NaK2Clcotransporter和Band3anionexchanger,但是对KClcotransporter无作用。

原价
¥6012-15275
价格
4810-12220
H100的二维码

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  • 货号: ajce45352
  • CAS: 643727-55-3
  • 别名:
  • 分子式: C18H16N2O6S
  • 分子量: 388.39
  • 纯度: >98%
  • 溶解度: Soluble in DMSO
  • 储存: Store at -20°C
  • 库存: 现货

Background

H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter and the Band 3 anion exchanger, but no effect against KCl cotransporter, in human erythrocytes.


H100 is a Cl- transport inhibitor, with partial effects against both the NaK2Cl cotransporter (NKCC) and the Band 3 anion exchanger (AE), but no effect against KCl cotransporter (KCC), in human erythrocytes. H100 (0.1 mM) shows 63% and 74% inhibition of NKCC and AE in human erythrocytes[1].


[1]. Culliford S1, et al. Specificity of classical and putative Cl(-) transport inhibitors on membrane transport pathways in human erythrocytes. Cell Physiol Biochem. 2003;13(4):181-8.

Protocol

Cell experiment:

35SO42- efflux is used as a convenient measure of the activity of the Band 3 anion exchanger (AE). Erythrocytes are suspended into MBS containing Na2SO4 rather than NaCl and incubated at 37°C for 30 min. The cell suspension is then centrifuged (3,000 g, 5 min) and the supernatant removed. The above procedure is repeated twice to ensure the intracellular replacement of Cl- with SO42-. To load the erythrocytes with radiolabel, the packed cells are re-suspended to approximately 50% haematocrit in a solution containing 1 part SO42- MBS and 9 parts of a medium containing (in mM) 300 sucrose and 10 MOPS (pH 7.4, 300 ± 5 mOsm) and placed in a microcentrifuge tube. 10 μCi of 35SO42- is then added and the suspension incubated at 37°C for 1 h. At the end of this period, the cells are then washed four times by centrifugation (10,000 g, 10 s) in ice-cold SO42- MBS. All inhibitors (H100) are dissolved in either DMSO or MBS and are added to the cell suspensions prior to the addition of the radioisotope[1].

参考文献:

[1]. Culliford S1, et al. Specificity of classical and putative Cl(-) transport inhibitors on membrane transport pathways in human erythrocytes. Cell Physiol Biochem. 2003;13(4):181-8.

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