Background
Syringaldehyde is a polyphenol that has been found in E. granulatus and has diverse biological activities.1,2,3,4 It inhibits LPS-induced COX-2 activity in RAW 264.7 cells with an IC50 value of 19.23 ?M.1 Syringaldehyde inhibits the proliferation of HCT116, Caco-2, and HT-29 colon cancer cells (IC50s = 56.3, 35.9, and 68.6 ?M, respectively).2 It decreases plasma glucose levels in a rat model of diabetes induced by streptozotocin when administered at doses ranging from 1.8 to 7.2 mg/kg.3 Syringaldehyde (25 and 50 mg/kg) reduces infarct size in a rat model of myocardial infarction induced by isoproterenol .4
1.Stanikunaite, R., Khan, S.I., Trappe, J.-M., et al.Cyclooxygenase-2 inhibitory and antioxidant compounds from the truffle Elaphomyces granulatusPhytother. Res.23(4)575-578(2009)
2.González-Sarrías, A., Li, L., and Seeram, N.P.Anticancer effects of maple syrup phenolics and extracts on proliferation, apoptosis, and cell cycle arrest of human colon cellsJ. Funct. Foods4(1)185-196(2012)
3.Huang, C.-H., Chen, M.-F., Chung, H.-H., et al.Antihyperglycemic effect of syringaldehyde in streptozotocin-induced diabetic ratsJ. Nat. Prod.75(8)1465-1468(2012)
4.Shahzad, S., Mateen, S., Mariyath, P.M.M., et al.Protective effect of syringaldehyde on biomolecular oxidation, inflammation and histopathological alterations in isoproterenol induced cardiotoxicity in ratsBiomed. Pharmacother.108625-633(2018)
Protocol
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Animal experiment:
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Rats[1]Adult male albino wistar rats (weighing 180-220?g)12.5?mg/kg, 25?mg/kg, and 50?mg/kg; p.o.; for 21 daysAdult male albino wistar rats are randomly divided into six groups (n=6), first group serves as control and receives vehicle (orally) for 21 days. Second group is given Syringaldehyde in saline orally at 50?mg/kg for 21 days. Third group receives vehicle for a period of 21 days and then ISO (100?mg/kg, s.c.) on 20th and 21?st day at an interval of 24?h. Fourth group is given Syringaldehyde for 21 days at 12.5?mg/kg, p.o. and ISO on 20th and 21?st day. Fifth group receives Syringaldehyde at concentration of 25?mg/kg, p.o. for 21 days and ISO on 20th and 21?st day. The sixth group is treated with 50?mg/kg of Syringaldehyde for 21 days and ISO on 20th and 21?st day. During the experimental procedure body weight of animals are monitored and on 22nd day, 24?h after second injection of ISO, rats sre sacrificed by cervical decapitation.Note: Body weight of animals belonging to different groups did not differed significantly but heart weight of ISO challenged animals were highly elevated (p?≤?0.05) than control rats. However, treatment of rats with Syringaldehyde significantly reduced heart weight in dose dependent way. The rats treated with Syringaldehyde alone displayed an insignificant difference from control group.
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参考文献:
[1]. Shahzad S, et al. Protective effect of syringaldehyde on biomolecular oxidation, inflammation and histopathological alterations in isoproterenol induced cardiotoxicity in rats. Biomed Pharmacother. 2018 Dec;108:625-633.
[2]. Stanikunaite R, et al. Cyclooxygenase-2 inhibitory and antioxidant compounds from the truffle Elaphomyces granulatus. Phytother Res. 2009 Apr;23(4):575-8.
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