A water-soluble vitamin
Thiamine is a water-soluble vitamin with antioxidant, neuroprotective, and anxiolytic properties.1,2,3,4 It inhibits lipid peroxidation in rat liver microsomes and free radical oxidation of oleic acid in vitro when used at concentrations ranging from 1 to 100 μM.1 In vivo, thiamine (100 mg/kg) reduces hepatic levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), lipid peroxidation, and protein damage in a rat model of acute ethanol intoxication.2 It reverses predator stress-induced suppression of hippocampal neurogenesis and decreases the latency of step-down from a platform, indicating anxiolytic-like activity in mice.3 Thiamine (8.5 mg/100 g food) reduces neurodegeneration and increases survival in Slc19a3-/- mice, a model of thiamine metabolism dysfunction syndrome-2 (THMD-2).4
1.Lukienko, P.I., Mel'nichenko, N.G., Zverinskii, I.V., et al.Antioxidant properties of thiamineBull. Exp. Biol. Med.130(9)874-876(2000) 2.Portari, G.V., Ovidio, P.P., Deminice, R., et al.Protective effect of treatment with thiamine or benfotiamine on liver oxidative damage in rat model of acute ethanol intoxicationLife Sci.16221-24(2016) 3.Vignisse, J., Sambon, M., Gorlova, A., et al.Thiamine and benfotiamine prevent stress-induced suppression of hippocampal neurogenesis in mice exposed to predation without affecting brain thiamine diphosphate levelsMol. Cell. Neurosci.82126-136(2017) 4.Suzuki, K., Yamada, K., Fukuhara, Y., et al.High-dose thiamine prevents brain lesions and prolongs survival of Slc19a3-deficient micePloS One12(6)e0180279(2017)
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Animal experiment: |
Slc19a3 E314Q KI mice are maintained routinely with conventional diet, which has a Thiamine concentration (thiamine hydrochloride, MW=337.3) of 1.71 mg/100 g food. Two types of Thiamine-restrict food base on “purified diets for laboratory rodents” are prepared, in which Thiamine concentration is 0.60 mg/100 g food (35% Thiamine of conventional food) or 0.27 mg/100 g food (16% Thiamine of conventional food). A high-Thiamine-containing food is also prepared from AIN-93M, in which Thiamine concentration is five times that of CE-2 (thiamine: 8.50 mg/100 g food)[2]. |
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参考文献: [1]. Kenneth Osiezagha, et al.Thiamine Deficiency and Delirium. Innov Clin Neurosci. 2013 Apr; 10(4): 26-32. |
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