现货大促销,价格低至8折起,量大更优惠,详细咨询客服
全部分类
全部分类
  • Transcrocetinate disodium (Disodium trans-crocetinate)
Transcrocetinate disodium (Disodium trans-crocetinate)的可视化放大

Transcrocetinate disodium (Disodium trans-crocetinate)

从藏红花 (Crocus sativus L.) 中提取的 Transcrocetinate disodium (Disodium trans-crocetinate) 作为 NMDA 受体拮抗剂具有高亲和力。

原价
¥1287-9850
价格
1030-7880
Transcrocetinate disodium (Disodium trans-crocetinate)的二维码

所有产品仅用于科学研究,我们不为任何个人用途提供产品和服务

询价有惊喜,量大更优惠 点击这里给我发消息

  • 库存: 现货
可选包装 >>>
首页
  • 货号: ajce49136
  • CAS: 591230-99-8
  • 别名: 藏红花酸二钠盐,Disodium trans-crocetinate
  • 分子式: C20H22Na2O4
  • 分子量: 372.37
  • 纯度: >98%
  • 溶解度: DMSO: < 1 mg/mL (insoluble or slightly soluble)
  • 储存: Store at -20°C
  • 库存: 现货

Background

Transcrocetinate disodium, extracted from saffron (Crocus sativus L.), acts as an NMDA receptor antagonist with high affinity.


Transcrocetinate (Transcrocetin, trans-Crocetin), a saffron metabolite originating from the crocin apocarotenoids, has been shown to exert strong NMDA receptor affinity and is thought to be responsible for the CNS activity of saffron.To ensure unchanged viability of Caco-2 cells throughout the transport experiments, cellular mitochondrial dehydrogenase activity of Caco-2 cells is measured by MTT assay after a 24 h incubation period with the test compounds: Hydroalcoholic saffron extract saffron extract (SE, 0.5-1 mg/mL) and crocin-1 (250-1000 µM) reveal no negative significant changes in cellular viability. Transcrocetinate at 10 µM level does not change viability while higher concentrations (40-160 µM) reduces significantly cellular viability[1].


[1]. Lautenschl?ger M, et al. Intestinal formation of trans-Crocetin from saffron extract (Crocus sativus L.) and in vitro permeation through intestinal and blood brain barrier. Phytomedicine. 2015 Jan 15;22(1):36-44.

Protocol

Cell experiment:

Cytotoxicity of test compounds is determined by MTT assay using Caco-2 cells in 96 well plates at a density of 20.000 cells per well in 200 μl FBS-free medium, grown for 96 h and followed by 24 h contact time with the test compounds (100 μL of serum-free media containing SE 0.5, 1, and 2 mg/mL; trans-crocin-1 250, 500, and 1000 μM; Transcrocetinate 10, 40, 80, and 160 μM) and incubation at 37°C/5% CO2. The incubation solutions are aspirated, each well is washed twice with 150 μL of PBS and 50 μL of MTT solution are added (2.5 mg/mL in PBS). Supernatants are discarded and the formed formazan is dissolved in 50 μL of DMSO. The absorption of the resulting solution is determined at λ=492 nm against reference wavelength λ=690 nm[1].

参考文献:

[1]. Lautenschl?ger M, et al. Intestinal formation of trans-Crocetin from saffron extract (Crocus sativus L.) and in vitro permeation through intestinal and blood brain barrier. Phytomedicine. 2015 Jan 15;22(1):36-44.

动态评分

0.0

没有评分数据
没有评价数据
一键回到顶部
展开 收缩
安捷凯在线客服