(-)-Catechin gallate ((-)-Catechin 3-gallate)|130405-40-2|安捷凯

Background

(–)-Catechin gallate ((–)-CG) is a catechin polyphenol that has been found in green tea extracts with diverse biological activities.^1,2,3,4 It inhibits proliferation of MDA-MB-231 breast cancer cells and reduces VEGF-induced tyrosine phosphorylation of VEGF receptor 2 (VEGFR2) in bovine aortic endothelial cells (BAECs) in a concentration-dependent manner.^1,2 (–)-CG is a competitive inhibitor of the facilitative glucose transporter GLUT4 (K_i = 90 μM) and inhibits methylglucose uptake by isolated rat adipocytes (IC₅₀ = 50 μM).³ It is also lytic against T. cruzi amastigotes, with a 50% bactericidal concentration (MBC₅₀) value of 48 pM.⁴

1.Lamy, S., Gingras, D., and Béliveau, R.Green tea catechins inhibit vascular endothelial growth factor receptor phosphorylationCancer Res.62(2)381-385(2002) 2.Sartippour, M.R., Heber, D., Ma, J., et al.Green tea and its catechins inhibit breast cancer xenograftsNutr. Cancer40(2)149-156(2001) 3.Strobel, P., Allard, C., Perez-Acle, T., et al.Myricetin, quercetin and catechin-gallate inhibit glucose uptake in isolated rat adipocytesBiochem. J.386(Pt 3)471-478(2005) 4.Paveto, C., Güida, M.C., Esteva, M.I., et al.Anti-Trypanosoma cruzi activity of green tea (Camellia sinensis) catechinsAntimicrob. Agents Chemother.48(1)69-74(2004)

Protocol

Cell experiment:

Human tongue squamous carcinoma (CAL27) cells and human salivary gland carcinoma (HSG) cells are used. Individual wells of a 96-well microtiter tissue culture plate are inoculated with 0.2 mL of the growth medium containing 2×104 cells/well for a 1-day exposure, 1.5×104 cells/ well for a 2-day exposure and 1×104 cells/well for a 3-day exposure to the test agents. After 1 day of incubation, the growth medium is removed and replaced with exposure medium, with or without varied concentrations of the test agents. In some studies the cells are coexposed to (-)-Catechin gallate (100, 200, 300, 400, and 500 μM) and 100 Units/mL catalase. After 1-3 days of exposure to the test agents, viability is assessed with the neutral red (NR) assay, which is based on the uptake and accumulation of the supravital dye, neutral red (NR)[1].

参考文献:

[1]. Babich H, et al. In vitro cytotoxicity of (-)-catechin gallate, a minor polyphenol in green tea. Toxicol Lett. 2007 Jul 10;171(3):171-80.