Glucoraphanin

A natural compound with protective effects

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5mg

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Background

Glucoraphanin is a natural glycoinsolate found in cruciferous vegetables, including broccoli.¹ It is converted to the isothiocyanate sulforaphane by the enzyme myrosinase.¹ Sulforaphane has powerful antioxidant, anti-inflammatory, and anti-carcinogenic effects.^1,2 It acts by activating nuclear factor erythroid 2-related factor 2 (Nrf2), which induces the expression of phase II detoxification enzymes.^3,4

1.James, D., Devaraj, S., Bellur, P., et al.Novel concepts of broccoli sulforaphanes and disease: Induction of phase II antioxidant and detoxification enzymes by enhanced-glucoraphanin broccoliNutr. Rev.70(11)654-665(2012) 2.Liang, H., and Yuan, Q.Natural sulforaphane as a functional chemopreventive agent: Including a review of isolation, purification and analysis methodsCrit. Rev. Biotechnol.32(3)218-234(2012) 3.La Marca, M., Beffy, P., Della Croce, C., et al.Structural influence of isothiocyanates on expression of cytochrome P450, phase II enzymes, and activation of Nrf2 in primary rat hepatocytesFood Chem. Toxicol.50(8)2822-2830(2012) 4.Dinkova-Kostova, A.T., Holtzclaw, W.D., Cole, R.N., et al.Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidantsProc. Natl. Acad. Sci. USA99(18)11908-11913(2012)

Protocol

Animal experiment:

Male C57BL/6JSlc mice are 7 weeks of age. The Nrf2 knockout (Nrf2?/?) mouse strain is used in the assay. After 1 week of acclimation, mice are fed normal chow (NC) (containing 2.2% dextrinized cornstarch, 10% kcal from fat), NC containing 0.3% glucoraphanin (NC-GR) (containing 2.2% extract powder), an HFD (containing 2.2% dextrinized cornstarch, 60% kcal from fat), or an HFD containing 0.3% glucoraphanin (HFD-GR) (containing 2.2% extract powder) for 14 weeks. Both the NC and the HFD containing cornstarch or glucoraphanin are prepared. All mice studied are maintained on a 12-h light/dark cycle at 24-26°C with free access to water and food.

参考文献:

[1]. Nagata N, et al. Glucoraphanin Ameliorates Obesity and Insulin Resistance Through Adipose Tissue Browning and Reduction of Metabolic Endotoxemia in Mice. Diabetes. 2017 May;66(5):1222-1236.
[2]. Yao W, et al. Role of Keap1-Nrf2 signaling in depression and dietary intake of glucoraphanin confers stress resilience in mice. Sci Rep. 2016 Jul 29;6:30659.