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Hesperin

An isothiocyanate with diverse biological activities

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¥1087-5875
价格
870-4700
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  • 货号: ajce50426
  • CAS: 4430-35-7
  • 别名: 6-MSITC
  • 分子式: C8H15NOS2
  • 分子量: 205.34
  • 纯度: >98%
  • 溶解度: DMSO : 50 mg/mL (243.50 mM)
  • 储存: Store at -20°C, protect from light, stored under nitrogen
  • 库存: 现货

Background

6-Methylsulfinylhexyl isothiocyanate (6-MSITC) is an isothiocyanate that has been found in wasabi (W. japonica) and has diverse biological activities.1,2,3,4,5 It inhibits nitric oxide (NO) production in mouse peritoneal exudate macrophages when used at a concentration of 5 ?M.1 It inhibits aggregation of isolated human platelets induced by arachidonic acid .2 6-MSITC reduces cell survival in a panel of breast, brain, colon, lung, ovarian, renal, and prostate cancer cell lines (mean IC50 = 43.7 ?M).3 In vivo, 6-MSITC (5 mg/kg twice weekly) reduces apoptosis of substantia nigral dopaminergic neurons and motor dysfunction in a mouse model of Parkinson's disease induced by 6-OHDA .4 6-MSITC also reduces the number of lung metastases in a B16/F10 murine melanoma model.5


1.Noshita, T., Kidachi, Y., Funayama, H., et al.Anti-nitric oxide production activity of isothiocyanates correlates with their polar surface area rather than their lipophilicityEur. J. Med. Chem.44(12)4931-4936(2009) 2.Morimitsu, Y., Hayashi, K., Nakagawa, Y., et al.Antiplatelet and anticancer isothiocyanates in Japanese domestic horseradish, WasabiMech. Ageing Dev.116(2-3)125-134(2000) 3.Nomura, T., Shinoda, S., Yamori, T., et al.Selective sensitivity to wasabi-derived 6-(methylsulfinyl)hexyl isothiocyanate of human breast cancer and melanoma cell lines studied in vitroCancer Detect. Prev.29(2)155-160(2005) 4.Morroni, F., Sita, G., Tarozzi, A., et al.Neuroprotection by 6-(methylsulfinyl)hexyl isothiocyanate in a 6-hydroxydopamine mouse model of Parkinson's diseaseBrain Res.158993-104(2014) 5.Fuke, Y., Shinoda, S., Nagata, I., et al.Preventive effect of oral administration of 6-(methylsulfinyl)hexyl isothiocyanate derived from wasabi (Wasabia japonica Matsum) against pulmonary metastasis of B16-BL6 mouse melanoma cellsCancer Detect. Prev.30(2)174-179(2006)

Protocol

Cell experiment:

Primary human umbilical vein endothelial cells (HUVECs) are cultured in collagen-coated tissue-culture dishes in an atmosphere containing 95 % air and 5 % CO2. Human monoblast U937 cells are grown in RPMI-1640 medium with 10 % fetal bovine serum, 10 U/mL Penicillin, and 10μg/mL Streptomycin. HUVECs are cultured in collagen-coated 96-well plates as confluent monolayers. Hesperin is added into wells at the indicated final concentrations (0-30 μg/mL) and then incubated for 24 h. Cell viability is measured by cell counting kits[2].

Animal experiment:

Mice[1]A colony of wild-type and Nrf2-null mice are backcrossed with C57BL/6 mice for ten generations. All mice are housed in the same animal care facility controlled for temperature, humidity, and light. Seven-week-old male wild-typeand Nrf2-null mice (n=6-8/group) are divided into five groups fed the following diets: 1) a standard diet (AIN-93, containing 4% soybean oil) for 12 weeks and vehicle (1:10 solution of DMSO/PBS) injected intraperitoneally 4 times per week for the last four weeks (control group), 2) a high-fat diet (HFD) (containing 4% soybean oil and 31% lard) for 12 weeks and vehicle injected intraperitoneally 4 times per week for the last four weeks (HFD group),3) a HFD for 12 weeks and Hesperin (10mg/Kg/day; dissolved in 1:10 solution of DMSO/PBS) injected intraperitoneally 4 times per week for the last four weeks (HFD+ Hesperin), 4) a HFD for 6 weeks followed by a HFD containing 1% carbonyl iron for 6 weeks and vehicle injected intraperitoneally 4 times per week for the last four weeks(HFD+Iron), and 5) a HFD for 6 weeks followed by a HFD containing 1% carbonyl iron for 6 weeks and Hesperin (10mg/Kg/day) injected intraperitoneally 4 times per week for the last four weeks (HFD+Iron+Hesperin). After 12 weeks, blood samples are collected by cardiac puncture under anesthesia with sodium pentobarbital (50 mg/kg, ip) and livers are harvested and stored at -80°C until analysis[1].

参考文献:

[1]. Tanaka Y, et al. 6-Methylsulfinylhexyl isothiocyanate prevents high-fat diet-induced fatty liver but fails to attenuate hepatic iron accumulation in mice. Clin Exp Pharmacol Physiol. 2016 Nov;43(11):1153-1156.
[2]. Okamoto T, et al. 6-Methylsulfinylhexyl isothiocyanate modulates endothelial cell function and suppresses leukocyte adhesion. J Nat Med. 2014 Jan;68(1):144-53.

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