A protein kinase substrate
Syntide-2 is a synthetic peptide recognized as a substrate by Ca2+/calmodulin-dependent protein kinase II (CaMKII; Km = 12 ?M).1 Its sequence is derived from the phosphorylation site 2 on glycogen synthase, a target of CaMKII action.1 This peptide has been used as a probe to assay CaMKII activity; however, protein kinase C, CaMKV, Raf-1, and other kinases are also known to recognize syntide-2 as a substrate.1,2,3,4
1.Hashimoto, Y., and Soderling, T.R.Calcium calmodulin-dependent protein kinase II and calcium phospholipid-dependent protein kinase activities in rat tissues assayed with a synthetic peptideArch. Biochem. Biophys.252(2)418-425(1987) 2.Inoue, S., Mizutani, A., Sugita, R., et al.Purification and characterization of a novel protein activator of Ca2+/calmodulin-dependent protein kinase IBiochem. Biophys. Res. Commun.215(3)861-867(1995) 3.Kumar, K.A., and Babu, P.P.CaM kinase II-alpha activity, levels and Ca/calmodulin dependent phosphorylation of substrate proteins in mice brain during fatal murine cerebral malariaNeurosci. Lett.336121-125(2003) 4.Force, T., Bonventre, J.V., Heidecker, G., et al.Enzymatic characteristics of the c-Raf-1 protein kinaseProc. Natl. Acad. Sci. USA911270-1274(1994)
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