Inulicin (1-O-Acetylbritannilactone)

A sesquiterpene lactone with diverse biological activities

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5mg

￥3737.00

2990.00

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10mg

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4280.00

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Background

1-O-Acetylbritannilactone is a sesquiterpene lactone that has been found in I. britannica and has diverse biological activities.^1,2 It inhibits LPS-induced production of prostaglandin E₂ and nuclear translocation of NF-κB in primary rat aorta vascular smooth muscle cells (VSMCs).¹ 1-O-Acetylbritannilactone (5 and 10 ?M) inhibits VEGF-induced capillary tube formation in human umbilical vein endothelial cells (HUVECs).² It reduces tumor growth in an A549 mouse xenograft model when administered at a dose of 12 mg/kg per day.

1.Liu, Y.-P., Wen, J.-K., Zheng, B., et al.Acetylbritannilactone suppresses lipopolysaccharide-induced vascular smooth muscle cell inflammatory responseEur. J. Pharmacol.577(1-3)28-34(2007) 2.Zhengfu, H., Hu, Z., Huiwen, M., et al.1-o-acetylbritannilactone (ABL) inhibits angiogenesis and lung cancer cell growth through regulating VEGF-Src-FAK signalingBiochem. Biophys. Res. Commun.464(2)422-427(2015)

Protocol

Cell experiment:

HUVECs or A549 cells are plated in 60 mm plates (300 cells/plate). After overnight incubation, cells are treated with applied agents (e.g., Inulicin; 5 μM and 10 μM) for 24 h. Cells are then washed, and fresh media are added. After 10 days of incubation, surviving colonies are fixed, stained, and manually counted[1].

Animal experiment:

Mice[1]Male nude mice (4-6weeks old, BALB/c) are used. A549 cells (five million cells in 0.1 mL of culture medium) are subcutaneously injected at the right thigh of nude mice, and treatment is started when the tumors reach an average volume about 100 mm3. Animals are randomized into two groups with 10 mice per group: (a) Vehicle; (b) 12 mg/kg of Inulicin. Inulicin is injected intraperitoneally (i.p.) daily. The mice are examined daily for toxicity/mortality relevant to treatment, and the tumor is measured with a caliper every two days. The tumor volume (in mm3) is calculated, and the tumor growth curve is presented. At the end of experiments, xenograft tumors are isolated through surgery and weighted[1].

参考文献:

[1]. Zhengfu H, et al. 1-o-acetylbritannilactone (ABL) inhibits angiogenesis and lung cancer cell growth through regulating VEGF-Src-FAK signaling. Biochem Biophys Res Commun. 2015 Aug 21;464(2):422-7.
[2]. Liu YP, et al. Acetylbritannilactone suppresses lipopolysaccharide-induced vascular smooth muscle cell inflammatory response. Eur J Pharmacol. 2007 Dec 22;577(1-3):28-34.