Amyloid Beta-Peptide (12-28) (human)
Amyloid Beta-Peptide (12-28)（人）是 Amyloid Beta 蛋白 (1-42) (Aβ (1-42)) 的肽片段。
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包装价格促销价数量
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1mg￥772.00694.00- +
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5mg￥2058.001852.00- +
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10mg￥3430.003087.00- +
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25mg￥5046.004541.00- +
- 货号: ajcp4340
- CAS: 107015-83-8
- 别名: 淀粉-Β-蛋白片段12-28,Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys
- 分子式: C89H135N25O25
- 分子量: 1955.2
- 纯度: ＞98%
- 溶解度: ≥ 195.5mg/mL in DMSO
- 储存: Desiccate at -20°C
- 库存: 现货
Background
Amyloid Beta-Peptide (12-28) (human) is a peptide fragment of amyloid beta protein (1-42) (Aβ (1-42))?[1]. One of the neuropathological features of Alzheimer's disease (AD) is the presence of amyloid deposits in senile plaques and in blood vessel walls?[2]. Amyloid Beta-Peptide (12-28) was used as an internal standard?[1].
Amyloid Beta-Peptide (12-28) (human) could efficiently suppress the formation of the α7nAChR.Aβ (1-42) complex. Using human brain tissues and cells that overexpress either the α7 nicotinic acetylcholine receptor (α7nAChR) or amyloid precursor protein as the starting material, Aβ (1-42) and α7nAChR can be co-immunoprecipitated by the respective specific antibodies, suggesting that they are tightly associated?[3].
参考文献:
[1]. Wang R, Sweeney D, Gandy SE, Sisodia, SS (1996) The profile of soluble amyloid b protein in cultured cell media. Detection and quantificaiton of amyloid β protein and variants by immunoprecipitation-mass spectrometry. J Biol Chem 271:31894-31902.
[2]. Terry R. D., and Davies P.(1983) Aging of the Brain (Samuel, D., Algeri, S.,Gershon ,S., Grimm, V., and Toffano, G., eds) Vol. 22, pp. 47-59, Raven Press
[3]. Wang, H. Y. et al. β-Amyloid1-42 binds to α7 nicotinic acetylcholine receptor with high affinity. Implications for Alzheimer's disease pathology. J. Biol. Chem. 275, 5626-5632 (2000).
Amyloid Beta-Peptide (12-28)（人）是 Amyloid Beta 蛋白 (1-42) (Aβ (1-42)) [1] 的肽片段。阿尔茨海默病 (AD) 的神经病理学特征之一是老年斑和血管壁中存在淀粉样沉积物 [2]。 Amyloid Beta-Peptide (12-28) 用作内标[1]。
Amyloid Beta-Peptide (12-28)（人）可有效抑制 α7nAChR.Aβ (1-42) 复合物的形成。使用过表达 α7 烟碱乙酰胆碱受体 (α7nAChR) 或淀粉样蛋白前体蛋白的人脑组织和细胞作为起始材料，Aβ (1-42) 和 α7nAChR 可以通过各自的特异性抗体进行免疫共沉淀，表明它们紧密结合关联的[3]。
Protocol
| Cell experiment [1]: | |
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Cell lines |
PC12 cells |
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Preparation Method |
Two days after plating cell were treated with different concentrations of aged β12-28 peptide. After 22 h, MTT (5 mg/ml) was added and incubation was continued for a further2 h. Cells were lysed and after 24 h at 37°C, colorimetric determination of MTT reduction was made at 550 nm. |
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Reaction Conditions |
0.001, 0.1, 10, 40 μM for 22 hours |
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Applications |
Freshly pre-pared solutions of β12 - 28 were non-toxic as expected, while aged solutions produce generally significant decreases in the MTT mitochondrial reduction by PC12 cells. |
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参考文献: [1]: Rabanal, F., Tusell, J. M., Sastre, L., et al. (2002) Structural, kinetic and cytotox-icity aspects of 12-28 β amyloid protein fragment: A reappraisal. J. Pept. Sci. 8,578-588. | |
